LIA

Autoimmune diseases are characterized by heterogeneous autoantibody patterns found in patient sera. Line immuno assays (LIA) are ideal autoimmune diagnostic tools for screening and differential diagnosis as well as monitoring autoimmune patients by identifying specific autoantibodies on a single strip. HUMAN’s autoimmune LIAs are developed for straight forward autoantibody profiling. LIAs are easy- to-use screening assays, employing disease-specific antigens. Up to 17 different autoantibodies can be differentiated with a single assay strip. This state-of-the art autoimmune diagnostic tool is produced by spotting antigens in lines on a nitrocellulose membrane. HUMAN utilizes the latest spotting technologies available in the market, including HUMAN’s patented epitope-preserving technology. In the assay, bound autoantibodies are visualized by using specific HRP-conjugated secondary antibodies that specifically target human autoantibodies and a LIA specific substrate. Quite unique is a cut-off line enabling for the easy determination of positive samples.

LIA Technology

Autoimmune diseases are characterized by autoanti-body patterns found in patient sera. HUMAN’s auto-immune line immune assays (LIAs) are developed for rapid autoantibody profiling. LIAs are simple to use screening assays. By employing disease-specific anti- gens, up to 17 different autoantibodies can be differ- entiated with a single-shot assay. This state-of-the-art autoimmune diagnostic tool is produced by spotting antigens in lines on a simple nitrocellulose membrane utilizing the newest technologies avail- able in the market, including HUMAN’s patented epitope-preserving technology. Bound autoantibodies are visualized by using specific HRP-conjugated sec-ondary antibodies that specifically target humanautoantibodies and a LIA specific substrate. Samples positive for autoantibodies can be easily detected by comparing the pattern of brown lines to the stan-dardized line (cut-off) that is also spotted on the LIAs. LIAs are ideal autoimmune diagnostic tools for screening and differential diagnosis as well as moni- toring autoimmune patients by identifying specific autoantibodies in one shot.

Mr. H and Differential Diagnosis in Autoimmune Diagnostics

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