Oncology
ONCOLOGY and EPIGENETICS
P. Zafiropoulos S.A. in collaboration Dia-Chem S.R.L. offers high specificity, sensitivity full panel systems, easy to perform for the study of oncology and epigenetics.
DNA methylation is a post-replicative process. The extention of the modifications regarding the DNA methylation is basically determined during development. DNA methylation is therefore one of the mechanisms related with cell differentiation through inhibition of gene expression at the transcriptional level and is essential for the normal development of mammals, it is associated with genomic imprinting, transcriptional inactivation of the X chromosome and aging, has a role in the development of pathological events, such as carcinogenesis. DNA methylation is an epigenetic post-synthetic modification that, with the transfer of a methyl group from Sadenosylmetionina C atom in position 5 of the cytosine ring, introduces 5mC as the new base in the DNA. The CpG islands contain CpG dinucleotides at a frequency mathematically predetermined. Most of the CpG islands are about 30,000 generally located 5 ‘end of the promoter region of housekeeping genes, sometimes overlapping the region coding for a variable extension (usually the first exon). The frequency of the presence the CpG dinucleotides in the genome is less than expected, except for the regions of CpG islands. This is the result of an evolutionary mechanism linked to the presence of a spontaneous deaminase activity in the nucleus. This enzymatic reaction transforms the methylated cytosine in thymine and the non-methylated C in Uracil. Checks and repair mechanisms recognize the Uracil base as extraneous DNA and therefore replace, while this substitution does not occur for Thymine, common base in the DNA. The most CpG are no methylated in a normal cell, regardless of the state of the transcriptional gene, while during cancer development the CpGs outside of CpG islands become hypomethylated, and CpG islands in the promoter region of tumor suppressor genes become hypermethylated. This hypermethylation is associated with chromatin condensation and loss of transcription. The most recent data indicate that genetic and epigenetic events interact to help the progressive development of cancers.
| Code | Description |
| 1.410 | Ampli set GSTP1 promoter Detection of methylation status of Glutatione-S-Transferase (GSTP1) promoter gene. With DNA modification |
| 1.411 | Ampli set Lung cancer Detection of methylation status of p16 (CDKN2), DAP-kinase and 06- metilguanine DNA methyltransferase (MGMT) promoter genes. With DNA modification |
| 1.412 | Ampli set Breast cancer Detection of methylation status of p16 (CDKN2), BRCA1 and GSTP1 promoter genes. With DNA modification |
| 1.413 | Ampli set Colon cancer Detection of methylation status of P16 (CDKN2) oncosuppressor gene, DAPkinase and 06-metilguanine DNA methyltransferase (MGMT) promoter genes.With DNA modification |
| 1.414 | Ampli set BRCA 1-2 Identification of 185delAG, 188del11bp, 5382insC BRCA1 and 6174delT BRCA2 genes mutations |
| 1.414-7 | Ampli set BRCA 1-2 Identification of 185delAG, 188del11bp, 5382insC, 1499insA, 5083del19 in BRCA1 and 6174delT, 5445del4 in BRCA2 genes mutations |
| 1.415 | Ampli set BRCA1 Identification of 1499insA, 3596del4, 4172insT, 5083del19, 677insA BRCA1 gene mutations |
| 1.416 | Ampli set BRCA2 Identification of 5445del4, 6696delTC, 9189del4 BRCA2 gene mutations |
| 1.417 | Ampli set p16 (CDKN2) promoter Detection of promoter methylation status in P16 (CDKN2) gene. With DNA modification |
| 1.419 | Ampli set MGMT promoter Detection of promoter methylation status in MGMT gene. With DNA modification |
| 1.420 | Ampli set hMLH 1 promoter Detection of promoter methylation status in hMLH1 gene. With DNA modification |
| 1.421 | Ampli set BRCA 1 promoter Detection of promoter methylation status in BRCA 1 gene. With DNA modification |
| 1.422 | Ampli set DAP-kinase promoter Detection of promoter methylation status in DAP kinase gene. With DNA modification |
| 1.429 |
Ampli set PARP-1 Val762Ala (Poly-ADP-ribosepolymerase-1) enzyme |
| ONCOLOGY EPIGENETICS REAL TIME PCR | |
| 1.410RT | Ampli set GSTP1 promoter Detection of methylation status of Glutatione-S-Transferase (GSTP1) promoter gene in real time PCR.With DNA modification |
| 1.417RT | Ampli set p16 (CDKN2) promoter Detection of methylation status of p16 promoter gene in real time PCR. With DNA modification |
| 1.419RT | Ampli set MGMT promoter Detection of methylation status of MGMT promoter gene in real time PCR. With DNA modification |
| 1.420RT | Ampli set hMLH 1 promoter Detection of methylation status of hMLH1 promoter gene in real time PCR. With DNA modification |
| 1.421RT | Ampli set BRCA 1 promoter Detection of methylation status of BRCA 1 promoter gene in real time PCR. With DNA modification |
| 1.422RT | Ampli set DAP-kinase promoter Detection of methylation status of DAP kinase promoter gene in real time PCR. With DNA modification |