DNA FLOW TECHNOLOGY
Vitro has a wide range of molecular diagnostic systems for the simultaneous detection of pathogen panels and markers, based on multiplex PCR or RT/PCR amplification and hybridization using DNA-FLOW technology.
It is an innovative technology that uses automated flow-through reverse hybridisation on porous membrane-based microarrays that contain oligonucleotides specific to each marker. The target DNA molecules cross the membrane through a vertical vacuum flow, facilitating their interaction with the specific and complementary DNA probes immobilised on the porous membrane in a three-dimensional environment, unlike conventional dot blot technology in which hybridization occurs passively. This accelerates the process and increases sensitivity, reducing reaction volumes and overall processing time from several hours to a few
minutes.
Once binding has occurred between the specific amplicons and their corresponding probes, this is visualised by a colorimetric immunoenzymatic reaction with Streptavidin-Alkaline Phosphatase and the BCIP/NBT chromogen, forming insoluble precipitates on the membrane in those areas where hybridisation has occurred. The process is performed automatically in the hybriSpot™ platform and the results are analysed with the hybriSoft™ software.
